Using Nanoparticles to Analyze Key Immune System Molecules in Embryonic Stem Cells

Figure 1: Labeling of Mouse Embryonic Stem Cells with Quantum Dot Nanoparticles

The goal of my IGERT thesis project is to characterize the expression and localization of MHC class I molecules in embryonic stem cells. Embryonic stem cells are pluripotent cells capable of unlimited self renewal as well as differentiation into all the cell types of the body. The major histocompatibility complex plays a major role in the success of transplantation therapy and is therefore an important area of research for ES cells and their differentiated derivatives. Semiconductor nanoparticles, quantum dots, will be utilized for specific imaging and localization of MHC class I proteins in embryonic stem cells and for live cell tracking of different stem cell populations.

Figure 2: Differentiating Embryonic Stem Cells (Embryoid Bodies)