Imaging of Embryonic Stem Cells using Multiphoton Luminescence of Gold Nanoparticles

Student: Paula Lampton
Department: Biology
Advisor: Carol Warner

Abstract

Using the Keck 3D Fusion Microscope (located at Northeastern University), we were able to detect gold nanoparticles via multiphoton photoluminescence in mouse embryonic stem cells. The embryonic stem cells were able to grow for several passages in the presence of the non-toxic gold nanoparticles and cellular uptake of the nanoparticles was demonstrated by multi-photon imaging. The use of multi-photon luminescence using gold nanoparticles may be useful for in vitro stem cell tracking and monitoring.